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KMID : 0380620160480030284
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Korean Journal of Food Science and Technology
2016 Volume.48 No. 3 p.284 ~ p.288
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Protective Effect of Ethyl Acetate Fraction from Hibiscus Sabdariffa L. Extract against High Glucose-induced Oxidative Stress
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Seung Tae-Wan
Park Sang-Hyun
Park Seon-Kyeong
Ha Jeong-Su
Lee Doo-Sang
Kang Jin-Yong
Kim Jong-Min
Lee Uk
Heo Ho-Jin
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Abstract
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To investigate the physiological effect of Hibiscus sabdariffa, in vitro antioxidant activities and neuroprotective effects against high glucose-induced oxidative stress were examined. The ethyl acetate fraction (EtOAc-Fr) from H. sabdariffa contained high total phenolic contents compared with other fractions but total anthocyanin contents were lower than 80% Ethanol extract showed the highest 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging activity and malondialdehyde inhibitory effect. Furthermore, the EtOAc-Fr decreased the intracellular reactive oxygen species level, and protected the neuron-like PC12 cells from high glucose-induced cytotoxicity. The EtOAc-Fr also presented inhibitory effects against acetylcholinesterase as an acetylcholine hydrolase enzyme. Finally, chlorogenic acids as main phenolics by high performance liquid chromatography analysis.
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KEYWORD
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high glucose, , chlorogenic acid, Hibiscus sabdariffa L.
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